ALDH1A3-acetaldehyde re-wires neural crest stem cell and high metabolism states to potentiate melanoma heterogeneity
Abstract
Cancer cellular heterogeneity and therapy resistance arise from metabolic and transcriptional adaptations, but how these are interconnected is less well understood. Here, we show that in melanoma, the cancer stem cell marker aldehyde dehydrogenase 1A3 (ALDH1A3) forms an enzymatic partnership with acetyl-CoA synthetase 2 (ACSS2) in the nucleus to couple high glucose metabolic flux with acetyl-histone H3 modification of neural crest lineage and glucose metabolism genes. Critically, we show acetaldehyde is a metabolite source for acetyl-histone H3 modification dependent on ALDH1A3, providing a physiologic function for this highly volatile and toxic metabolite. In a zebrafish model of melanoma residual disease, a subpopulation of ALDH1A3-high cells emerges following BRAF inhibitor treatment and targeting these with an ALDH1 suicide inhibitor, nifurtimox, delays or prevents BRAF inhibitor drug-resistant relapse. Our work reveals that ALDH1A3-ACSS2 directly coordinates local acetaldehyde-acetyl-CoA metabolism with specific chromatin-based gene regulation and represents a potential therapeutic vulnerability in melanoma.