In multicellular organisms, stem cells are impacted by microenvironmental resources such as nutrient availability and oxygen tension for their survival, growth, and differentiation 1,2 . However, the acessibility of these resources in the pericellular environment greatly varies from organ to organ 3–5 . This divergence in resource availability leads to variations in the potency and differentiation potential of stem cells 4,6 . Moreover, hexose and oxygen levels modulate cytokine production which is crucial for cell-cell communication 7,8 as well as growth, and differentiation of stem cell 9,10 . Hence, this study aims to explore the distinct effects of glucose and fructose, as well as different oxygen tensions, on the growth dynamics, cytokine production, and differentiation of stem cells.