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Preprints

Identification of the immune infiltration landscape and development of the prognostic signature on cuproptosis-related genes in colorectal cancer

Kang Y, Zhao S, Chang X, Huang J, Wang J, Chen Y.
Preprint from
Research Square
11 May 2022
PPR
PPR491491
Abstract

Background:

Cuproptosis is a novel mechanism of cell death induced by extra copper that was directly bound with the lipoylated proteins in the TCA cycle and could lead to the dysfunction of the mitochondrion. However, the impact of cuproptosis on TIME in colorectal cancer remains unclear. The investigation of the interplay between cuproptosis and TIME could help us decipher novel strategies for colorectal cancer diagnosis and treatment. This study aimed to construct a prognostic signature on immunotherapies and explore the novel patterns of the cuproptosis-related genes(CRGs) in colorectal cancer.

Methods:

: All the analyses were performed by R packages. The biological roles and functional patterns of the CRGs were performed by GO/KEGG analysis. The signature was developed with the prognostic CRGs by LASSO COX regression analysis. TIMER algorithm was selected for the immune cell infiltration analysis and the OCLR algorithm was for calculating the stem cell index. Consensus analysis was used to explore the novel landscape of the CRGs in colorectal cancer.

Results:

: The CRGs mainly participated in the process of the TCA cycle and energy metabolism in colorectal cancer. Five CRGs were identified with prognostic values and the correlation with immune infiltration was also demonstrated, meanwhile, two genes could be independent biomarkers for immunotherapies. By consensus analysis, the CRGs also showed a significant difference in stem cell characteristics.

Conclusion:

A comprehensive analysis of functional and immune infiltration of CRGs in colorectal cancer was performed. The results also provided evidence for revealing the vulnerabilities in the correlation between cuproptosis and TIME. More data from research trials and experiments are required.