Lung adenocarcinoma is a cancer with a high mortality rate and a low survival rate. Ferroptosis can play an important role in anti-tumor immunity and tumor suppression. NCOA4, as a selective cargo receptor for ferritin autophagy turnover, is very important for Ferroptosis. However, NCOA4 used as a biomarker for the diagnosis, prognosis and treatment of lung adenocarcinoma remains unclean. Therefore, we use ONCOMINE, Tumor Immune Estimation Resource, UALCAN, GEPIA, TNMplot, Human Protein Atlas, Kaplan-Meier plotter, OncoLnc, GeneMANIA, STRING, LinkedOmics database to determine the relationship between NCOA4 expression, prognosis and immunoprecipitation in lung adenocarcinoma. Finally, we used OCLR to calculate mRNA expression-based stemness index of LUAD patient’s data in TCGA and analyzed the correlation between NCOA4 and markers of lung cancer stem cells. We obtained the low expression of NCOA4 in lung adenocarcinoma. Moreover, low expression of NCOA4 is positively correlated with poor overall lifetime. Studies based on the TIMER database show that NCOA4 has significant positive correlations with a variety of tumor immune cells, in particular CD8 + T cells, macrophages cells, neutrophil cells, and dendritic cells. Furthermore, we used the LinkedOmics database to evaluate gene co-expression of NCOA4 in LUAD and to investigate their role in tumor immunity and ferroptosis. And we found that NCOA4 is closely related to surface markers of lung cancer stem cells. Therefore, NCOA4 may be a biomarker for predicting the survival and diagnosis of LUAD.