Autophagy is a conserved process that degrades cytoplasmic components and organelles in metazoan cells including germline stem cells. Although autophagy is implicated in the aging of stem cells, the precise mechanism are still unknown. Here we show that elevating autophagy by overexpressing (OE) Drosophila Autophagy-related gene 8a (Atg8a) in the female Germline stem cells (GSCs) delays their loss due to aging. However, sustained elevated autophagy levels in old flies promote GSC loss due to cell death. In contrast, knockdown of Atg8a ( Atg8aRNAi ) in GSCs accelerates their loss. Atg8a OE GSCs show elevated autophagy flux, and increased mitotic activity even at 8 weeks of age. Atg8a OE GSCs possess smaller-sized mitochondria and exhibit reduced mitochondrial oxidative stress in the GSCs. However, in contrast Atg8aRNAi GSCs have elevated mitochondrial ROS and possess larger mitochondria. Finally, our data show that Atg8a OE GSCs occupy the stem cell niche for longer duration with the aid of elevated E-cadherin at the GSC-cap cell contact sites. Our data suggests that elevated autophagy promotes GSC maintenance and activity, and delays their aging.

Graphical abstract