Spermatogonia Stem Cells (SSCs) are potential candidates for reprogramming, therapeutic neuronal repair, and regeneration. Recent studies have revealed that differentiated cells can be reverted to a pluripotent state by overexpressing a set of pluripotent transcription factors. Oct4 (encoded by pou5f1), a POU transcription factor family member, is essential to the potential that controls pluripotency, and it is widely expressed in pluripotent stem cells, although it decreased or suppressed after differentiation. In this investigated research, we examined the Oct4 Expression in vivo and in-vitro of SSCs, Embryonic Stem Cell-like (ES-like), Embryonic Bodies (EBs), and Neurons by Immunocytochemistry (ICC), Immunohistochemistry (IMH), and Fluidigm Real-Time polymerase chain reaction. In addition, We use some databases like STRING to predict protein-protein interaction and enrichment analysis. We evaluated the expression of Oct4 in this process, and we observed that it is expressed in SSCs, ES-like, and EBs during the differentiation of spermatogonia stem cells into adult neurons. We show that by adding RA to EBs, the Expression of Oct4 is reduced and is not expressed in the neuron cells. We observed that the Expression of Oct4 is linked and interacts with the differentiation of spermatogonia stem cells into neuron cells, and it has been shown to be biological functional like stem cell maintenance and somatic cell reprogramming. Our findings can help us better understand the process of differentiation of spermatogonia stem cells into neurons, and it can be effective in finding new and more efficient treatments for neurogenesis and repair of neurons. Moreover, this study may comprehend the new Oct4 reprogramming method for clinical and therapeutic use.